Modelo experimental de endometriose em ratas: caracterização e validação por meio de análise histológica e enzimática

  • Fabiana Albani Zambuzi
  • Maria Luiza Nunes Mamede Rosa
  • Andrea Carla Celotto
Palavras-chave: Endometriose, neutrófilo, inflamação, ratas, modelo experimental

Resumo

Introdução: A endometriose é uma doença caracterizada pela presença de tecido endometrial fora da cavidade uterina, que acomete um número expressivo de mulheres em idade reprodutiva e apresenta um diagnóstico complexo e invasivo. Objetivo: Caracterizar e validar um modelo experimental de endometriose descrito na literatura utilizando ratas Wistar. O estudo avaliou i) a habilidade em realizar o procedimento de indução da endometriose em ratas e assegurar a reprodutibilidade do modelo; ii) a viabilidade de obter amostras histopatológicas dos implantes que permitam caracterizar a doença. Material e métodos: A endometriose foi induzida em ratas adultas que tiveram um fragmento do corno uterino retirado e implantado na cavidade peritoneal. Após 21 dias, os implantes foram extraídos para realização das análises histológicas e dosagem de mieloperoxidase (MPO). Resultados: Foi demonstrado que houve aderência do implante ao peritônio, levando a formação de aderência das alças intestinais, tecido adiposo abdominal, cistos e novos vasos sanguíneos. A partir da análise histológica, observou-se o desenvolvimento característico do endométrio compatível com a fase de estro, em que há presença de células cúbicas com núcleo evidente e infiltrado de neutrófilos nos implantes, além da detecção de MPO. Conclusão: O modelo empregado é de fácil execução e boa reprodutibilidade, capaz de reproduzir as características morfológicas da endometriose, além de possibilitar a obtenção de amostras para avaliação das alterações funcionais de células do sistema imune, bem como a detecção de mediadores produzidos por estas células que também estão relacionados à patogênese da endometriose.

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Publicado
2020-12-30
Seção
Ciências Biológicas